Glutamate Receptor 1 anticorps (pSer831)

N° du produit ABIN5688924

Cet anticorps Lapin Polyclonal détecte spécifiquement Glutamate Receptor 1 dans WB. Il présente une réactivité envers Humain, Rat et Souris.

Aperçu rapide pour Glutamate Receptor 1 anticorps (pSer831) (ABIN5688924)

Antigène: Glutamate Receptor 1 (GLUR1)

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Glutamate Receptor 1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Glutamate Receptor 1 anticorps

Épitope: pSer831

Specificité: GluR1 antibody is specific for GluR1 phosphorylated at Ser831.

Purification: Affinity Purified

Immunogène: GluR1 (Ser831) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser831 of rat GluR1.

Information d'application

Indications d'application: Immunolabeling of the GluR1 protein in Western blots of rat brain is blocked by the Ser831 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100 % amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Stock: -20 °C

Stockage commentaire: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Détails sur Glutamate Receptor 1

Antigène: Glutamate Receptor 1 (GLUR1)

Autre désignation: GluR1

Sujet: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α,-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct subunits GluR1-4 and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is also potentiated by phosphorylation at Ser831. In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory.

Poids moléculaire: 100 kDa

ID gène: 50592

UniProt: P19490

Pathways: Signalisation PI3K-Akt